Fig. 1. Obesity is associated with SIRT3 down-regulation in HF patients. Tissue samples from non-obese (NOB) and obese (OB) patients with HF were isolated and protein expression of SIRT3 (A) and acetylation profile (B) was analyzed. GAPDH was used as a reference of protein expression. Correlation between (C) SIRT3 and acetylation profile, (D) BMI and acetylation profile, and (E) SIRT and BNP was performed. The data are presented as mean ± SEM from NOB (n=6) and OB (n=5) patients. A t-test was performed to obtain the statistical analysis.